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Rare but serious reactions include chest pain, irregular heartbeat, or paradoxical bronchospasm (worsening wheeze), all of which require immediate medical attention

After incubation, wash the wells 5 times with washing buffer, blot dry, and add 100L of a 1:1 mixture of substrate A and B to each well

We dont aim for a standard medical diagnosis such as IBD or diverticulitis, we get to the core of why the gut dysfunction is occurring

Most available data comes from in vitro experiments and animal models, forming an early foundation for ongoing investigation